Reference genome, PacBio-HiFi, Muscle tissue

Tenebrionidae, Promethis sterrha, USYD-Pro2, Beetle, Project Lead: Nathan Lo

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This data is made available openly under a Creative Commons Attribution license. Please see the initiative Data Policy for attribution information.

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Field Value
Geospatial Coverage

Dataset extent

Resource Permissions organization_member_after_embargo:date_of_transfer_to_archive:365:tsi-consortium-members
Access Control Date 2026-07-23
Access Control Mode date
Sequence Data Type pacbio-hifi
access_rights restricted
ala_specimen_url https://bie.ala.org.au/species/https://biodiversity.org.au/afd/taxa/425c1804-cd3f-42ad-b8a9-189a324c238b
analysis_software SMRTLink 25.1.0.257715
base_url https://downloads-qcif.bioplatforms.com/bpa/tsi_staging/pacbio-hifi/BPAOPS-1846/20250718_TSI_BRF_m84118_250718_062134_s1/
bioplatforms_project Threatened Species Initiative
cell_postion 1_C01
certainty Certain
class Insecta
collection_date 2024-09-17
collection_method Hand collected
collector Nathan Lo, Simon Ho, Younis Menkara
country Australia
data_context Reference genome
data_custodian Nathan Lo
data_type PacBio-HiFi
dataset_id 102.100.100/629205
date_of_transfer 2025-07-23
date_of_transfer_to_archive 2025-07-23
decimal_latitude_public -31.5
decimal_longitude_public 159.0
dna_treatment DNA sheared with Megaruptor, followed by Ligation of Twist universal adapter and amplified using 16-UDI index H01
facility_project_code PacBio_WGS52_965
facility_sample_id 965-3
family Tenebrionidae
file_type bam
flowcell_id m84118_250718_062134_s1
flowcell_type Revio SMRT Cells 25M
folder_name 20250718_TSI_BRF_m84118_250718_062134_s1
genus Promethis
insert_size_range 9118.0
institution_name the University of Sydeny
latitude -31.5
library_comments AmpliFi ultra-low PacBio methods
library_construction_protocol AmpliFi
library_id 102.100.100/683176
library_index_id bc2063
library_index_id_dual bc2063
library_index_seq_dual TCTGCATCATGAGTAT
library_index_sequence TCTGCATCATGAGTAT
library_location BRF labs
library_ng_ul 45.2
library_pcr_cycles 10.0
library_prep_date 2025-07-16
library_prepared_by Xiangning Liu
library_source Genomic
library_strategy WGS
library_type pacbio-hifi
lifestage Adult organism
location_text Blackburn Island
longitude 159.0
material_conc_ng_ul 23.4
material_extracted_by Yu-Hsiang Ho
material_extraction_date 2025-05-07
material_extraction_method Monarch HMW DNA Extraction Kit, Sodium Acetate precipitate
material_extraction_type DNA
metadata_revision_date 2025-08-26
metadata_revision_filename 20250826_Sample_metadata_COMBINED-FOR-PORTAL_NOLATLON.xlsx
method_of_determination Dissection
movie_length 24.0
n_libraries_pooled 2.0
order Coleoptera
phenotypic_sex Female
sample_custodian Yu-Hsiang Ho, Nathan Lo
sample_id 102.100.100/671146
sample_quality Suspect some DNA degradation
sample_type Tissue sample
scientific_name Promethis sterrha
scientific_name_authorship (Olliff, 1889)
scientific_name_note NA
sequencing_facility BRF
sequencing_kit_chemistry_version SPRQ
sequencing_model Revio
sequencing_platform PacBio
species sterrha
specimen_id USYD-Pro2
specimen_id_description University of Sydney MEEP lab institutional ID
state_or_region New South Wales
subspecies NA
taxonomic_group Beetle
ticket BPAOPS-1846
tissue_collection Entomology
tissue_collection_type Living collection
tissue_number Pro2-2 2-3Pre
tissue_preservation Refrigerate , elution buffer
tissue_preservation_temperature 4.0
tissue_type Muscle tissue
type_status unknown
wild_captive Wild
work_order 14104