Trichothecium, Reference Genome, Illumina-transcriptomics, Trichothecium tissue growing on stripe rust

Hypocreales incertae sedis, Trichothecium NA, PCB_RNA_IS3, Fungi, Project Lead: John Rathjen

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Data and Resources

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Additional Info Show Blank Fields

Field Value
Resource Permissions organization_member_after_embargo:date_of_transfer_to_archive:365:fungi-consortium-members
Access Control Date 2026-05-30
Access Control Mode date
Sequence Data Type illumina-shortread
altitude NA
analysis_software Illumina DRAGEN BCLConvert 4.3.16
ancillary_notes RNA isolated from the hyperparasite growing on stripe rust spores as a form of nutrition
associated_media Images of colonies available
bait_set_name N/A
bait_set_reference N/A
base_url https://downloads-qcif.bioplatforms.com/bpa/fungi_staging/illumina-shortread/BPAOPS-1790/20250530_FUN_BRF_467821_22KMT3LT3/
bioplatforms_dataset_id 102.100.100/467821
bioplatforms_library_id 102.100.100/467150
bioplatforms_project Australian Functional Fungi Initiative
bioplatforms_sample_id 102.100.100/465098
ccg_jira_ticket BPAOPS-1790
cell_postion N/A
class Sordariomycetes
collection_date 2022-03-17
collection_method Environment: collected rust infected leaves, kept at high humidity to promote growth then subcultured fluffy growth until a pure culture was obtained
collector Jack Wess (ANU)
common_name Trichothecium
country Australia
data_context Reference Genome
data_type Illumina-shortread
date_of_transfer 2025-05-30
date_of_transfer_to_archive 2025-06-02
decimal_latitude_public NA
decimal_longitude_public NA
depth NA
dna_treatment N/A
env_broad_scale outdoor envrionment
env_local_scale Poplar trees surrounding a sports field and near a riverbank on a university campus
env_medium Poplar rust infected Poplar leaf
experimental_design N/A
facility_project_code NA
facility_sample_id 467150_FUN_BRF_22KMT3LT3_CTTATTGGCC-GATAACAAGT
family Hypocreales incertae sedis
fast5_compression N/A
flow_cell_id 22KMT3LT3
flowcell_id 22KMT3LT3
flowcell_type NovaSeq X Series 10B
folder_name 20250530_FUN_BRF_467821_22KMT3LT3
genus Trichothecium
habitat Australian National University Campus
health_state NA
host_common_name Poplar rust
host_family Melampsoraceae
host_organ NA
host_scientific_name Melampsora medusae
host_status Wild
host_symptom Pink fluffy growth
identified_by Jack Wess (ANU)
indigenous_location Ngunnawal and Ngambri land
insert_size_range 381.0
isolate Sample discovered growing on Poplar rust pustules in planta
library_comments N/A
library_construction_protocol Illumina DNA Prep
library_id 467150
library_index_id UDP0141
library_index_id_dual UDP0141
library_index_seq_dual GATAACAAGT
library_index_sequence CTTATTGGCC
library_layout Paired end
library_location BRF Freezer
library_ng_ul 0.757
library_oligo_sequence CAAGCAGAAGACGGCATACGAGATcttattggccCTGTCTCTTATACACATCTCCGAGCCCACGAGAC
library_oligo_sequence_dual AATGATACGGCGACCACCGAGATCTACACgataacaagtTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG
library_pcr_cycles 12.0
library_pcr_reps N/A
library_prepared_by Lachlan Morrison
library_selection N/A
library_source Total RNA
library_strategy Tagmentation
library_type Illumina-transcriptomics
life_stage Other (mature hyperparasite and prey spore)
location_info_restricted NA
location_text The Australian National University, 46 Sullivans Creek Rd, Acton ACT 2601
material_conc_ng_ul 115.2
material_extracted_by Jack Wess
material_extraction_date 2025-03-06
material_extraction_method Qiagen Rneasy Plant Mini Kit
material_extraction_type total RNA
metadata_revision_date 2025-08-25
metadata_revision_filename FUN_MASTER_sample_metadata_FORDP_20250825_NOLATLON.xlsx
model_base_caller N/A
movie_length N/A
n_libraries_pooled 1.0
order Hypocreales
phylum Ascomycota
project_collaborators Jack Wess
project_lead John Rathjen
sample_collection_type wild
sample_custodian Jack Wess, Australian National University
sample_id PCB_RNA_IS3
sample_quality Highly pure
sample_type mixed culture
scientific_name Trichothecium sp.
scientific_name_authorship Jack Wess
scientific_name_note All identifications are to genus level and identified via sequencing the ITS barcode region of the fungus
sequencing_facility BRF
sequencing_kit_chemistry_version N/A
sequencing_model NovaSeq X Series
sequencing_platform Illumina
source_population NA
species NA
specimen_custodian Jack Wess, Australian National University
specimen_id PCB_RNA_IS3
specimen_id_description Short-hand identifier used by researcher
state_or_region Australian Capital Territory
sub_species NA
taxon_id NA
taxonomic_group Fungi
temperature 22.0
ticket BPAOPS-1790
tissue Trichothecium tissue growing on stripe rust
tissue_preservation 25% glycerol stock of pure Trichothecium spores
tissue_preservation_temperature -80.0
type_status NA
wild_captive NA
work_order 21009